Nature2019Anzalone et al.The paper introducing prime editing: writing new sequence directly into the genome.
Research paper · Method
Search-and-replace genome editing without double-strand breaks or donor DNA
The paper introducing prime editing: writing new sequence directly into the genome.
简单说明
This showed a way to write chosen new DNA sequence into a specific place, without cutting both strands and without needing a separate template molecule. In principle it can make far more kinds of change than base editing can.
深入了解
Anzalone, Randolph, Davis, Sousa, Koblan, Levy, Chen, Wilson, Newby, Raguram and Liu described prime editing, in which a Cas9 nickase fused to a reverse transcriptase, directed by a pegRNA carrying both the target specification and the replacement template, writes new sequence directly onto the genomic strand. The paper reported all 12 base-to-base conversions plus small insertions and deletions.