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Nature2019Anzalone et al.The paper introducing prime editing: writing new sequence directly into the genome.
Research paper · Method
Search-and-replace genome editing without double-strand breaks or donor DNA
The paper introducing prime editing: writing new sequence directly into the genome.
간단한 설명
This showed a way to write chosen new DNA sequence into a specific place, without cutting both strands and without needing a separate template molecule. In principle it can make far more kinds of change than base editing can.
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Anzalone, Randolph, Davis, Sousa, Koblan, Levy, Chen, Wilson, Newby, Raguram and Liu described prime editing, in which a Cas9 nickase fused to a reverse transcriptase, directed by a pegRNA carrying both the target specification and the replacement template, writes new sequence directly onto the genomic strand. The paper reported all 12 base-to-base conversions plus small insertions and deletions.