🧭 Guided View New to genetics? We explain every term as you browse, in plain English. Same pages, with the help built in.
⚡ Expert View You already know the biology. Just the content — clean and compact, no extra explanations. This is the default view.
Interface language
Light mode
Research paper · Method
Search-and-replace genome editing without double-strand breaks or donor DNA
The paper introducing prime editing — writing new sequence directly into the genome.
Simple explanation
This showed a way to write chosen new DNA sequence into a specific place, without cutting both strands and without needing a separate template molecule. In principle it can make far more kinds of change than base editing can.
Go deeper
Anzalone, Randolph, Davis, Sousa, Koblan, Levy, Chen, Wilson, Newby, Raguram and Liu described prime editing, in which a Cas9 nickase fused to a reverse transcriptase, directed by a pegRNA carrying both the target specification and the replacement template, writes new sequence directly onto the genomic strand. The paper reported all 12 base-to-base conversions plus small insertions and deletions.
Educational information only
This page is a reference, not medical advice. Research and regulatory status change; check the last-updated date above and confirm anything important against the primary sources listed.