Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
The paper that introduced base editing — chemistry instead of cutting.
简单说明
This paper showed you could change a single DNA letter without cutting the double helix at all, by attaching a chemical enzyme to a disabled CRISPR protein. It removed the most dangerous step in gene editing.
深入了解
Komor, Kim, Packer, Zuris and Liu fused a cytidine deaminase to catalytically impaired Cas9, converting C•G to T•A within a small editing window without generating double-strand breaks, and improved efficiency by including a uracil glycosylase inhibitor and a nickase.