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Nature2016Komor et al.The paper that introduced base editing: chemistry instead of cutting.
Research paper · Method
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
The paper that introduced base editing: chemistry instead of cutting.
Simple explanation
This paper showed you could change a single DNA letter without cutting the double helix at all, by attaching a chemical enzyme to a disabled CRISPR protein. It removed the most dangerous step in gene editing.
Go deeper
Komor, Kim, Packer, Zuris and Liu fused a cytidine deaminase to catalytically impaired Cas9, converting C•G to T•A within a small editing window without generating double-strand breaks, and improved efficiency by including a uracil glycosylase inhibitor and a nickase.
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